InSite® Semen Detection Kit
Finally, a comparative study was conducted between seven different brands of PSA strips, as well as an RSIDTM semenogelin test, using the same semen dilution series as before. The results are shown in Fig 4. Strips A-E, G and H are PSA, while F and I are semenogelin. There were notable differences between brands. The "E" brand was chosen for inclusion in the InSite kit because it seemed to have the best sensitivity and readability. A few QC problems were noted with the "B" strips, which otherwise were identical with "E". The RSID test ("I") did not work well under these conditions, probably because the required buffer solutions were not used to run the test, but plain water was used instead. In contrast, the semenogelin strips from company "F" worked just fine.
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1/1,000
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1/1,000
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1/1,000
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1/1,000
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1/1,000
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1/1,000
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1/1,000
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1/1,000
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A
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B
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C
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D
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E
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G
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H
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I
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1/10,000
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1/10,000
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1/10,000
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1/10,000
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1/10,000
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1/10,000
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1/10,000
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1/10,000
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1/10,000
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A
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B
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C
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D
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E
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F
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G
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H
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I
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1/50,000
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1/50,000
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1/50,000
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1/50,000
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1/50,000
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1/50,000
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1/50,000
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1/50,000
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A
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B
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C
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D
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E
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G
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H
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I
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1/100,000
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1/100,000
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1/100,000
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1/100,000
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1/100,000
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1/100,000
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1/100,000
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1/100,000
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1/100,000
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A
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B
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C
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D
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E
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F
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G
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H
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I
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1/250,000
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1/250,000
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1/250,000
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1/250,000
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1/250,000
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1/250,000
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A
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B
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D
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E
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G
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H
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1/500,000
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1/500,000
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1/500,000
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1/500,000
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1/500,000
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1/500,000
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A
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B
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D
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E
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G
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H
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1/1,000,000
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1/1,000,000
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1/1,000,000
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1/1,000,000
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1/1,000,000
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1/1,000,000
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1/1,000,000
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A
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B
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C
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D
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E
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G
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H
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|
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1/2,000,000
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1/2,000,000
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1/2,000,000
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1/2,000,000
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1/2,000,000
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B
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D
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E
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G
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H
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|
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1/4,000,000
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1/4,000,000
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1/4,000,000
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1/4,000,000
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1/4,000,000
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B
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D
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E
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G
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H
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Figure 4. Serial dilutions of semen and comparative testing between brands of PSA and semenogelin strips. A = PSA; B = PSA; C = PSA; D = PSA; E = PSA; F = Semenogelin; G = PSA; H = PSA; I = Independent Forensics RSID (semenogelin). Note: PSA strips from Company "B" and Company "E" look identical.
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