The sensitivity of PSA strips from various manufacturers was measured using a similar dilution series, and a semenogelin immunochromatographic test was used simultaneously for comparison.  The results are shown in Fig 3.  Strips A, B and D are PSA, while strip B is semenogelin.  Both tests proved to be sensitive down to a dilution of 1/500,000 which is generally consistent with that previously reported [Ref. 9,10]. Since semenogelin strips were ten times more expensive than PSA strips, the latter were chosen for inclusion in the InSite kit.  The PSA strips were also judged to have better visibility than the semenogelin strips.

 

 

1/1,000	1/1,000	1/1,000	1/1,000
A	B	C	D

 

1/10,000	1/10,000	1/10,000	1/10,000
A	B	C	D

 

1/100,000	1/100,000	1/100,000	1/100,000
A	B	C	D

 

1/500,000	1/500,000	1/500,000	1/500,000
A	B	C	D

 

1/1,000,000	1/1,000,000	1/1,000,000	1/1,000,000
A	B	C	D

 

1/2,000,000	1/2,000,000	1/2,000,000	1/2,000,000
A	B	C	D

 

 

Figure 3.  Serial Dilutions of Semen and Analysis with PSA Strips and Semenogelin Strips from various Manufacturers
A= PSA;  B= Semenogelin;  C= PSA;  D= PSA.  Note:  PSA strips from Company "A" and Company "C" look identical and raw materials probably come from the same distributor.